To solve this problem, we developed two strategies, both using oldenlandia affinis asparaginyl endopeptidases 1 with mutation C247A (abbreviated as OaAEP1, or AEP) for enzymatic ligation. 20 First, a heterofunctional peptide-based crosslinker, glycine–leucine (GL)-peptide-propargylglycine (Pra) was designed.

Aug 30, 2022 · First, we designed a heterobifunctional peptide-based crosslinker, GL-peptide-propargylglycine, which links to an N 3 -functionalized surface via the click reaction. Then, the target protein with a C-terminal NGL sequence can be immobilized via the AEP-mediated ligation.

Sep 4, 2021 · A secreted aldose 1-epimerase (AEP1) was shown to induce cell death in Nicotiana benthamiana, as did the other two AEP1s from different Phytophthora species. AEP1 could also trigger immune responses in N. benthamiana, other Solanaceae plants, and Arabidopsis (Arabidopsis thaliana).

Studies on sunflower AEP1 (HaAEP1), a predominant hydrolase, showed that a catalytic cysteine to serine mutation abolished enzyme activity, a catalytic histidine to alanine mutation drastically reduced activity, and a catalytic asparagine to alanine mutation resulted in a higher transpeptidation to hydrolysis ratio .

Phytophthora sojae apoplastic effector AEP1 triggers pattern-triggered immunity in nonhost plants and contributes to P. sojae virulence by promoting the uptake of extracellular sugar. In nature, plants are challenged by multiple potential pathogenic microbes.

May 11, 2023 · Cyclic and linear molecules of varying sizes and charges were employed to probe the radius and chemical environment of the pore, providing deep insights for expected future endeavors at predicting the structure of octameric Aep1. γ-CD showed unique suitability as an 8-subunit adapter in octameric Aep1, enabling the discrimination of β ...

Nov 8, 2021 · Methods to efficiently and site-specifically conjugate proteins to nucleic acids could enable exciting application in bioanalytics and biotechnology. Here, we report the use of the strict protein ligase to covalently ligate a protein to a peptide nucleic acid (PNA).

Aug 30, 2022 · To solve this problem, we simplified the procedure using two strategies both based on asparaginyl endopeptidase (AEP). First, we designed a heterobifunctional peptide-based crosslinker,...

10 μL Aep1 (0.4 mg/mL) was mixed with 10 μL mannan (1.5 mg/mL) at pH 5.5, and incubated at room temperature for 1 h. A lipid solution of 30 mg/mL was prepared by using 1, 2-diphytanoyl-sn-glycero-3-phosphocholine in

ATPase expression protein 1 (AEP1) is a yeast mitochondrial protein. It is essential for the expression of subunit 9 of mitochondrial ATP synthase.