Both Smac +/+ and Smac −/− mice died within 3 or 10 h after injection of 100 or 10 μg of anti-Fas antibody, respectively, with similar kinetics (Fig. 5A). Hepatocyte apoptosis was verified by staining liver sections with H&E (Fig. a, c, and e) or TUNEL (Fig. b, d, and f).

Sep 13, 2022 · SMAC-KO A549 cell-showed inhibited tumor growth and proliferation and activated apoptosis. The small SMAC-depleted “tumor” showed a morphology of alveoli-like structures, reversed epithelial-mesenchymal transition, and altered tumor microenvironment.

Apr 1, 2010 · Smac has been shown to bind to XIAP, cIAP-1 and cIAP-2 and Smac mimetics sensitize tumors to programmed cell death (7, 9–11). In this series of experiments, we explore the effect of a novel Smac mimetic, JP1201, in combination with chemotherapy.

Jun 5, 2024 · Despite being pro-apoptotic, SMAC/Diablo is overexpressed in and required for tumor development. We identified and produced SMAC-interacting sequences in several proteins as cell-penetrating peptides that disrupt cancer cells’ SMAC-essential functions.

May 5, 2013 · In this study, we investigated the role of SMAC in intestinal tumorigenesis using both human samples and animal models. Decreased SMAC expression was found to correlate with increased cIAP2 expression and higher grades of human colon cancer.

Sep 24, 2024 · Background: We have engineered a Smac-armed oncolytic virus by inserting a Smac transgene into the genome of a vesicular stomatitis virus to generate VSV-S. Our previous study shows that the anticancer efficacy of VSV-S is more potent than that of wild-typed VSV in a subcutaneous TNBC mouse model.

Nov 1, 2010 · We found that sulindac given for only 1 wk markedly induced apoptosis detected by TUNEL staining in the small intestinal crypts of APCMin/+ mice, with 22.1% of crypts containing at least one TUNEL-positive cell, compared with only 4.0% in mice receiving control diet (Fig. 1 A).

SMAC/Diablo, a pro‐apoptotic protein, yet it is overexpressed in several cancer types. We have described a noncanonical function for SMAC/Diablo as a regulator of lipid synthesis during cancer cell proliferation and development. Here, we explore the ...

Apoptosis was assessed by terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling (TUNEL). Smac/DIABLO expression levels in experimental and control groups were examined by immunohistochemistry and Western blot. Results: Khat significantly exacerbates the neurological damage compared with control (p<0.05).

Aug 1, 2020 · These phenomena may explain why apoptotic GBC cells were observed in the HE staining, TUNEL assay, and cleaved caspase-3 immunohistochemistry results of the Smac group. This result may be due to the secretion of the inflammatory factor TNF-α by tumor cells during growth or chemotherapy.