Jul 6, 2022 · We developed a system to produce stable HIV-1 Pol and determined its cryo–electron microscopy structure. RT in Pol has a similar arrangement to the mature RT heterodimer, and its dimerization may draw together two PR …

May 29, 2013 · The structure of the HIV-1 capsid is analysed by cryo-electron microscopy and cryo-electron tomography, allowing presentation of an all-atom molecular dynamics model of the entire capsid.

Mar 29, 2021 · Here, we present single-particle cryo-EM structures of CD4-mimetics BNM-III-170 and M48U1 bound to a BG505 native-like Env trimer plus the CD4-induced antibody 17b at 3.7 Å and 3.9 Å...

Oct 31, 2013 · Infection of host cells by HIV-1 is mediated by an envelope glycoprotein (Env) trimeric spike on the surface of the virus. Proteins comprising the Env trimer must be cleaved for infectivity, and thus viral fusion involves three Env conformations.

The cryo-EM structure of a soluble cleaved Env SOSIP gp140 trimer in complex with broadly neutralizing antibody PGV04 reveals key features that dictate how Env functions and how it interacts with the human immune system.

Dec 20, 2013 · The HIV-1 envelope glycoprotein (Env) trimer contains the receptor binding sites and membrane fusion machinery that introduce the viral genome into the host cell. As the only target for broadly neutralizing antibodies (bnAbs), Env is a focus for rational vaccine design. We present a cryo-electron mi …

To visualize the Env-PG16 complex directly, we have determined by cryo-EM, at 4.6 Å resolution, the structure of a fully processed, full-length, cell-surface expressed Env, isolated directly by binding with affinity tagged PG16 Fab before extracting the complex from the cell membranes.

Mar 4, 2016 · Here we present the cryo-EM reconstruction of a cleaved wild-type JR-FL EnvΔCT trimer in complex with PGT151 Fab at 4.2 Å resolution, demonstrating the structure not only of wild-type Env but also of a type I viral fusion protein with an intact TM, which unexpectedly was found to be flexible.

Mar 4, 2016 · The envelope glycoprotein trimer (Env) on the surface of HIV-1 recognizes CD4(+) T cells and mediates viral entry. During this process, Env undergoes substantial conformational rearrangements, making it difficult to study in its native state. Soluble stabilized trimers have provided valuable insight …

Here, we present single-particle cryo-EM structures of CD4-mimetics BNM-III-170 and M48U1 bound to a BG505 native-like Env trimer plus the CD4-induced antibody 17b at 3.7 Å and 3.9 Å resolution, respectively.